![]() ![]() However, if extracted samples exhibit inhibition, addition of bovine serum albumin to PCR reagents will overcome this problem. caespitum The most effective method to prevent inhibition before DNA extraction was to exude crop contents and crop structures onto UV-sterilized tissue. We found significant differences in the frequency of inhibition between the three species tested, with inhibition most evident in T. Following dissection, we determined that within the gaster, the digestive tract and crop cause significant levels of inhibition. No body segment, other than the gaster, caused significant inhibition. ![]() We designed a series of experiments to determine those ant organs responsible for inhibition and identified variation in inhibition among three species (Tetramorium caespitum (L.), Solenopsis invicta Buren, and Camponotus floridanus (Buckley)). Although this method can provide insight, its use on ants has been limited, potentially due to inhibition when amplifying gut content DNA. Molecular gut content analysis provides a means of delineating food web linkages of ants based on the presence of prey DNA within their guts. Generalist predators play an important role in many terrestrial systems, especially within agricultural settings, and ants (Hymenoptera: Formicidae) often constitute important linkages of these food webs, as they are abundant and influential in these ecosystems. Penn, Hannah J Chapman, Eric G Harwood, James D Overcoming PCR Inhibition During DNA-Based Gut Content Analysis of Ants. ![]()
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